The approach used a light-sensitive proton channel from the fungus; essentially a “switch” allowed the researchers to trigger acidification and measure the cellular response. Experiments with Arabidopsis thaliana demonstrated persistent depolarization and acidification that persisted even after light stimulation was discontinued; This indicates the activation of the plant’s own ion channels.
The study also found that acidification causes a calcium signal lasting up to 200 seconds that emanates from the endoplasmic reticulum, the internal calcium store.
Source: Ferra

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